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Palavra-chave Immobilization (54) Biodegradation (3) Lipase (3) Saccharomyces cerevisiae (3) Alginate (2) Anaerobiosis (2) Cyclodextrin glucanotransferase (2) Ethanol (2) Morphometry (2) Response surface methodology (2) Β-galactosidase (2) (hyper)thermophilic marine micro-organisms (1) 16S rRNA (1) 5.01.00.00-9 Agronomia biomassa microbiana (1) ATPase (1) Acetonitrile (1) Activity (1) Activity and Stability (1) Adsorption (1) Adubos fosfatados (1) Mais... Tipo do documento Info:eu-repo/semantics/article (39) Journal article (9) Text (3) Info:eu-repo/semantics/other (1) Preprint (1) Ano 2019 (1) 2018 (3) 2017 (4) 2016 (9) 2015 (2) 2014 (5) 2013 (3) 2012 (2) 2011 (2) 2010 (1) 2009 (2) 2008 (5) 2007 (1) 2006 (6) 2005 (2) 2004 (4) 2000 (1) 1998 (1) Mais... País Brazil (40) Chile (9) France (3) Belgium (1) Mexico (1) Idioma Inglês (52) Espanhol (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 54 Primeira ... 1 2 3 ... Última Decreased spermatogenic and androgenic testicular functions in adult rats submitted to immobilization-induced stress from prepuberty BJMBR Almeida,S.A.; Petenusci,S.O.; Anselmo-Franci,J.A.; Rosa-e-Silva,A.A.M.; Lamano-Carvalho,T.L.. We investigated whether chronic stress applied from prepuberty to full sexual maturity interferes with spermatogenic and androgenic testicular functions. Male Wistar rats (40 days old) were immobilized 6 h a day for 60 days. Following immobilization, plasma concentrations of corticosterone and prolactin increased 135% and 48%, respectively, while plasma luteinizing hormone and testosterone presented a significant decrease of 29% and 37%, respectively. Plasma concentration of follicle-stimulating hormone was not altered in stressed rats. Chronic stress reduced the amount of mature spermatids in the testis by 16% and the spermatozoon concentration in the cauda epididymidis by 32%. A 17% reduction in weight and a 42% decrease in DNA content were observed in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Spermatogenesis; LH; FSH; Testosterone; Prolactin. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100013 Biodegradation of acetonitrile by cells of Candida guilliermondii UFMG-Y65 immobilized in alginate, kappa-carrageenan and citric pectin BJM Dias,João Carlos T.; Rezende,Rachel P.; Linardi,Valter R.. Different encapsulation matrices were tested for immobilized cells of Candida guilliermondii UFMG-Y65 used for acetonitrile degradation. Acetonitrile degradation by free cells and cells immobilized in Ba-alginate, kappa-carrageenan and citric pectin was studied. The rate of acetonitrile degradation was monitored for 120 h by measuring yeast growth and ammonia concentration. Different alginate concentrations did not affect cell viability, but the period of incubation in BaCl2 solution reduced the number of viable cells. Likewise, the gel nature and the matrix structure of the support resulting from the cell immobilization conditions were of fundamental importance for biocatalyst activity and performance, affecting substantially the patterns of microbial... Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida guilliermondii; Acetonitrile; Biodegradation; Immobilization. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100015 Sulfated fucan as support for antibiotic immobilization BJMBR Araújo,P.M.; Oliveira,G.B.; Córdula,C.R.; Leite,E.L.; Carvalho Jr.,L.B.; Silva,M.P.C.. Xylofucoglucuronan from Spatoglossum schröederi algae was tested as a support for antibiotic immobilization. The polysaccharide (20 mg in 6 ml) was first activated using carbodiimide, 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide methiodide (20 mg in 2 ml), under stirring for 1 h at 25ºC and pH from 4.5 to 5.0. After adjusting the pH to 8.0, either gentamicin or amikacin (62.5 mg in 1.25 ml) was then immobilized on this chemically modified polysaccharide with shaking for 24 h in a cold room. Infrared spectra of the activated carbodiimide xylofucoglucuronan showed two bands to carbonyl (C = O at 1647.9 and 1700.7 cm-1) and to amide (C<FONT FACE=Symbol>Ý</FONT>-NH2) groups (1662.8 and 1714.0 cm-1). Microbial characterization of the derivatives... Tipo: Info:eu-repo/semantics/other Palavras-chave: Sulfated fucan; Immobilization; Antibiotics; Gentamicin; Amikacin. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300002 Effect of one stretch a week applied to the immobilized soleus muscle on rat muscle fiber morphology BJMBR Gomes,A.R.S.; Coutinho,E.L.; França,C.N.; Polonio,J.; Salvini,T.F.. We determined the effect of stretching applied once a week to the soleus muscle immobilized in the shortened position on muscle fiber morphology. Twenty-six male Wistar rats weighing 269 ± 26 g were divided into three groups. Group I, the left soleus was immobilized in the shortened position for 3 weeks; group II, the soleus was immobilized in the shortened position and stretched once a week for 3 weeks; group III, the soleus was submitted only to stretching once a week for 3 weeks. The medial part of the soleus muscle was frozen for histology and muscle fiber area evaluation and the lateral part was used for the determination of number and length of serial sarcomeres. Soleus muscle submitted only to immobilization showed a reduction in weight (44 ± 6%, P... Tipo: Info:eu-repo/semantics/article Palavras-chave: Muscle; Stretching; Immobilization; Sarcomere number; Muscle fiber area. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000005 Thiocyanate degradation by pure and mixed cultures of microorganisms BJM Souza-Fagundes,Elaine M.; Rosa,Luiz H.; Gomes,Newton C.M.; Santos,Mércia H.; Pimentel,Patricia F.. A mixed culture and a pure bacterial strain (BMV8) were isolated from a bioreactor for thiocyanate treatment. Both cultures removed 5 mM of thiocyanate from the medium in 36 hours. The mixed culture was able to tolerate concentrations up to 60 mM. The efficiency of thiocyanate degradation decreased when the cells were immobilized. Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Cyanate; Immobilization; Microorganisms; Thiocyanate. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300012 Stability of free and immobilized Lactobacillus acidophilus and Bifidobacterium lactis in acidified milk and of immobilized B. lactis in yoghurt BJM Grosso,Carlos Raimundo Ferreira; Fávaro-Trindade,Carmen Sílvia. This study evaluated the stability of Bifidobacterium lactis (Bb-12) and of Lactobacillus acidophilus (La-05) both free and immobilized in calcium alginate, in milk and in acidified milk (pH 5.0, 4.4 and 3.8). The stability of immobilized B. lactis in yoghurt (fermented to pH 4.2), during 28 days of refrigerated storage was also evaluated. The efficiency of two culture media (modified MRS agar and Reinforced Clostridial Agar plus Prussian Blue) for counting of B. lactis in yoghurt was determined. Lee's agar was used to count Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus when B. lactis were counted in the MRS medium. B. lactis and L. acidophilus in both free and immobilized forms presented satisfactory rates of survival in milk... Tipo: Info:eu-repo/semantics/article Palavras-chave: Probiotics; Immobilization; Alginate; Lactobacillus acidophilus; Bifidobacterium lactis; Yoghurt; Milk; Lactobacillus bulgaricus. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100025 45-and 70-base DNA supramolecular polymerizations on quartz crystal microbalance biosensor ArchiMer Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Antoine, Elisabeth; Compere, Chantal. Supramolecular polymerizations of 45- and 70- base DNAs on the surface of an in-situ time-resolved 27 MHz quartz crystal microbalance biosensor. Tipo: Text Palavras-chave: Sensor; Design; Kinetics; Immobilization; Hybridization. Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1710.pdf A comparative study on fungal laccases immobilized on chitosan BABT Araújo,José Hilton Bernardino de; Uemura,Vinicius Oliveira; Moraes,Flavio Faria de; Barbosa,Aneli de Melo; Zanin,Gisella Maria. The phenoloxidase enzyme laccase from the cultures of the Pleurotus ostreatus and Botryosphaeria sp. and a commercial laccase from Aspergillus sp. were immobilized on chitosan of pharmaceutical degree by adsorption followed by crosslinking. Different immobilization conditions in relation to the granulometry of support and amount of enzymatic laccase extract used were tested, aiming at reaching high enzymatic activity with the immobilized enzyme. Two different substrates, ABTS and DMP, were used for the determination of enzymatic activity. The highest enzymatic activity was obtained when 1.0mg/mL of the enzymatic laccase extract from Botryosphaeria sp. was used with 1.0g of support (200 mesh). These immobilized enzymes are to be applied to the improvement... Tipo: Info:eu-repo/semantics/article Palavras-chave: Laccases; Immobilization; Chitosan. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400001 Immobilization and stability studies of a lipase from thermophilic Bacillus sp: The effect of process parameters on immobilization of enzyme Electron. J. Biotechnol. Nawani,Neerupma; Singh,Rajvinder; Kaur,Jagdeep. A thermostable lipase was partially purified from the culture supernatant of a thermophilic Bacillus sp. The enzyme is optimally active at 60&ordm;C and pH 8.0. The enzyme showed enhancement in activity in presence of benzene or hexane (30% v/v each). The activity (assayed by determining the release of pNP from pNP laurate) was stimulated up to 60% of these solvents in enzyme reaction mixture. The catalytic properties of this thermostable enzyme can be further improved via the use of different immobilization techniques and reaction conditions. Enzyme was immobilized on different solid supports and their enzyme activity and stability was compared. The enzyme was adsorbed on silica and HP-20 beads followed by cross-linking with gluteraldehyde on HP-20,... Tipo: Journal article Palavras-chave: Esterification; Immobilization; Lipase; Thermostability. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500011 Utilización de la κ-carragenina como soporte de inmovilización de microorganismos marinos degradadores de hidrocarburos. OceanDocs Fonseca, E. L.; Núñez, R. R.; Villaverde, M. J; Barbán, O. M.; Ferrer, O. B.; Ramos, I.; Valdés, O.. Una marea negra provocada por el vertido deliberado o accidental de crudos de petróleo, aceite combustible, fangos o residuos petrolíferos y productos refinados, provoca un gran impacto sobre los ecosistemas marinos y costeros. En este trabajo, se desarrolla un producto compuesto por bacterias marinas inmovilizadas por atrapamiento en k- carragenina semirrefinada y en cuya formulación no se incluyen nutrientes. Los resultados muestran que la variante de inmovilización más ventajosa se obtuvo cuando fue empleada la κ-carragenina al 1% gelificada con una solución de KCL 1.5 M. Mediante análisis de Cromatografía Gaseosa de Alta Resolución y de espectroscopia (FTIR), se demostró la alta capacidad de degradación del bioproducto frente a petróleo del Yacimiento... Tipo: Preprint Palavras-chave: Immobilization; Oil; Immobilization; Http://aims.fao.org/aos/agrovoc/c_27550. Ano: 2006 URL: http://hdl.handle.net/1834/3662 Effect of raffinose and ultrasound pulses on invertase release by free and immobilized Saccharomyces cerevisiae in loofa (Luffa cylindrica) sponge BABT Marques,Leila Larisa Medeiros; Buzato,João Batista; Celligoi,Maria Antonia Pedrine Colabone. This study investigated the effect of raffinose and ultrasound pulses on invertase release from free S. cerevisiae and S. cerevisiae immobilized in Luffa cylindrica. The free cell culture was submitted to 2% raffinose pulse and irradiated for 2 minutes at 0.12 and 0.46 h-1 dilution rates. The immobilized cell culture was submitted to raffinose pulse and irradiated for 1, 2 and 4 minutes, at 0.10 h-1 dilution rate. In immobilized cells, the raffinose pulse increased the invertase activity from 5.38 to 7.27 U/mg. Ultrasound application in free cell culture at the 0.12 h-1 dilution rate gave the best results. The activity varied from 25.08 to 29.38 U/mg while the increase in immobilized cells was from 5.22 to 9.70 U/mg when sonicated for two minutes. These... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Invertase; Luffa cylindrica; Raffinose; Saccharomyces cerevisiae; Ultrasound. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700003 The production of sorbitol by permeabilized and immobilized cells of Z. mobilis in sucrose BABT Vignoli,Josiane Alessandra; Celligoi,Maria Antonia Colabone; Silva,Rui Sérgio Ferreira da; Barros,Márcio de. The production of sorbitol by permeabilized and immobilized cells of Zymomonas mobilis in Luffa cylindrica was investigated in sucrose medium. A full 2³ factorial design was used to verify the influence of each factor and its interactions. The cell permeabilization showed a significant and negative effect upon the production of sorbitol, while the time of cultivation and the immobilization process were significant and positive. The results demonstrated that the cell immobilization and the time of cultivation of 36 h presented higher production of sorbitol. Tipo: Info:eu-repo/semantics/article Palavras-chave: Permeabilization; Immobilization; Sucrose; Z. mobilis. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500020 Rat hindlimb joint immobilization with acrylic resin orthoses BJMBR da Silva,C.A.; Guirro,R.R.J.; Polacow,M.L.O.; Cancelliero,K.M.; Durigan,J.L.Q.. The objective of the present study was to propose an orthosis of light material that would be functional for the animal and that would maintain only the ankle joint immobilized. Male Wistar rats (3 to 4 months old, 250-300 g) were divided into 2 groups (N = 6): control and immobilized for 7 days. Rats were anesthetized with sodium pentobarbital (40 mg/kg weight) and the left hindlimb was immobilized with the orthoses composed of acrylic resin model, abdominal belt and lateral supports. The following analyses were performed: glycogen content of the soleus, extensor digitorum longus, white gastrocnemius, red gastrocnemius, and tibialis anterior muscles by the phenol sulfuric method, and the weight, fiber area and intramuscular connective tissue of the soleus... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Hypotrophy; Joint; Orthoses; Physical therapy. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700016 Cyclosporin-A does not affect skeletal muscle mass during disuse and recovery BJMBR Aoki,M.S.; Miyabara,E.H.; Soares,A.G.; Salvini,T.F.; Moriscot,A.S.. Cyclosporin-A (CsA) is an immunosuppressive drug that acts as an inhibitor of calcineurin, a calcium phosphatase that has been suggested to play a role in skeletal muscle hypertrophy. The aim of the present study was to determine the effect of CsA administration (25 mg kg-1 day-1) on skeletal muscle mass and phenotype during disuse and recovery. Male Wistar rats received vehicle (N = 8) or CsA (N = 8) during hind limb immobilization (N = 8) and recovery (N = 8). Muscle weight (dry/wet) and cross-sectional area were evaluated to verify the effect of CsA treatment on muscle mass. Muscle phenotype was assessed by histochemistry of myosin ATPase. CsA administration during immobilization and recovery did not change muscle/body weight ratio in the soleus (SOL)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Calcineurin; Immobilization; Recovery; Skeletal muscle; Hypertrophy; Cyclosporin-A. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200011 Maize response to nitrogen fertilization timing in two tillage systems in a soil with high organic matter content Rev. Bras. Ciênc. Solo Sangoi,Luis; Ernani,Paulo Roberto; Silva,Paulo Regis Ferreira da. No-tillage systems, associated to black oat as preceding cover crop, have been increasingly adopted. This has motivated anticipated maize nitrogen fertilization, transferring it from the side-dress system at the stage when plants have five to six expanded leaves to when the preceding cover crop is eliminated or to maize sowing. This study was conducted to evaluate the effects of soil tillage system and timing of N fertilization on maize grain yield and agronomic efficiency of N applied to a soil with high organic matter content. A three-year field experiment was conducted in Lages, state of Santa Catarina, from 1999 onwards. Treatments were set up in a split plot arrangement. Two soil tillage systems were tested in the main plots: conventional tillage (CT)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Nitrogen; Immobilization; Leaching; Grain yield. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832007000300011 Amplitude no fósforo microbiano em um Argissolo em pastagem nativa submetida à roçada e à introdução de espécies forrageiras com fertilização fosfatada em diferentes épocas - DOI: 10.4025/actasciagron.v30i4.5319 Agronomy Santos, Danilo Rheinheimer; UFSM; Martinazzo, Rosane; UFRGS; Gatiboni, Luciano Colpo; UDESC; Kaminski, João; UFSM; Silva, Leandro Souza da; UFSM. Em solos com elevado grau de intemperismo, a imobilização temporária de fósforo pela biomassa microbiana do solo (BMS) pode ser um mecanismo de retardamento das reações de adsorção. O objetivo do presente trabalho foi avaliar a amplitude no fósforo microbiano (Pm) em um Argissolo em pastagem nativa submetida à roçada, introdução de forrageiras exóticas e adubadas com fertilizantes fosfatados. O estudo foi desenvolvido a partir de um experimento instalado em 1997 que constava de cinco tratamentos de fosfato e calagem (testemunha sem fósforo; hiperfosfato de gafsa; superfosfato triplo; superfosfato triplo + calcário e superfosfato simples + calcário), distribuídos em quatro blocos ao acaso. Em junho de 2002, a pastagem nativa remanescente foi roçada, semeada... Palavras-chave: 5.01.00.00-9 Agronomia biomassa microbiana; Imobilização; Adubos fosfatados; Pastagens nativas; Disponibilidade de fósforo. microbial biomass; Immobilization; Phosphate fertilizers; Natural pasture; Phosphorus availability. Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5319 Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles BABT Bruno,Laura Maria; Lima Filho,José Luiz de; Castro,Heizir Ferreira de. Microbial lipase from Mucor miehei and Candida rugosa were immobilized by covalent binding onto magnetized polysiloxane polyvinyl alcohol particles (POS-PVA). The resulting immobilized derivatives were evaluated in aqueous solution (olive oil hydrolysis) and organic solvent (butyl butyrate synthesis). Higher catalytic activities were found when the coupling procedure was made with M. miehei lipase. Immobilized M. miehei lipase also showed a better operational stability and a higher half-life than C. rugosa lipase after the successive batches of esterification. The performance of C. rugosa immobilized derivative was constrained by the low lipase loading used in the immobilizing step. Further information regarding the both immobilized derivatives was also... Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida lipase; Mucor lipase; Hybrid matrix; Immobilization. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500003 Hydrolysis of whey lactose by immobilized β-Galactosidase BABT Mariotti,Marcela Panaro; Yamanaka,Hideko; Araujo,Angela Regina; Trevisan,Henrique Celso. Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-galactosidase; Immobilization; Lactase; Whey; Lactose; Hydrolysis. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019 Histomorphometric analysis of the response of rat skeletal muscle to swimming, immobilization and rehabilitation BJMBR Nascimento,C.C.F.; Padula,N.; Milani,J.G.P.O.; Shimano,A.C.; Martinez,E.Z.; Mattiello-Sverzut,A.C.. The objective of the present study was to determine to what extent, if any, swimming training applied before immobilization in a cast interferes with the rehabilitation process in rat muscles. Female Wistar rats, mean weight 260.52 ± 16.26 g, were divided into 4 groups of 6 rats each: control, 6 weeks under baseline conditions; trained, swimming training for 6 weeks; trained-immobilized, swimming training for 6 weeks and then immobilized for 1 week; trained-immobilized-rehabilitated, swimming training for 6 weeks, immobilized for 1 week and then remobilized with swimming for 2 weeks. The animals were then sacrificed and the soleus and tibialis anterior muscles were dissected, frozen in liquid nitrogen and processed histochemically (H&E and mATPase).... Tipo: Info:eu-repo/semantics/article Palavras-chave: Swimming training; Female rat skeletal muscles; Immobilization; Morphometry; ATPase. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900013 The effect of a low dose of clenbuterol on rat soleus muscle submitted to joint immobilization BJMBR Cancelliero,K.M.; Durigan,J.L.Q.; Vieira,R.P.; Silva,C.A.; Polacow,M.L.O.. The aim of the present study was to evaluate the effect of joint immobilization on morphometric parameters and glycogen content of soleus muscle treated with clenbuterol. Male Wistar (3-4 months old) rats were divided into 4 groups (N = 6 for each group): control, clenbuterol, immobilized, and immobilized treated with clenbuterol. Immobilization was performed with acrylic resin orthoses and 10 µg/kg body weight clenbuterol was administered subcutaneously for 7 days. The following parameters were measured the next day on soleus muscle: weight, glycogen content, cross-sectional area, and connective tissue content. The clenbuterol group showed an increase in glycogen (81.6%, 0.38 ± 0.09 vs 0.69 ± 0.06 mg/100 g; P < 0.05) without alteration in weight,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Clenbuterol; Connective tissue; Glycogen content; Morphometry; Rat soleus muscle. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200003 Registros recuperados: 54 Primeira ... 1 2 3 ... Última

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